BVD CONSULT

Diagnostic assays applied in BVDV control in The Netherlands

Animal Health Service, P.O. Box 9, 7400 AA Deventer, The Netherlands

Abstract

In The Netherlands, a voluntary bovine  virus  diarrhoea  virus (BVDV)-free certification programme was started in 1997. After an intake procedure in which all cattle are tested for the presence of  BVD  virus, a herd obtains the status “ BVD -virus-free”. To maintain this status a monitoring procedure is executed to verify absence of BVDV circulation in the herd. Several diagnostic tests are used: RT-PCR in bulk milk and pooled blood samples, antigen-ELISA (Ag-ELISA) and antibody ELISA in individual blood samples. Sensitivity and specificity of these tests are discussed. In addition, a diagnostic quick scan has been introduced, consisting of a combination of bulk milk tests for virus and antibody, and antibody tests in samples from young stock. Preliminary results are presented.

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This article was published in Preventive Veterinary Medicine, 72, M.H. Mars, and C. Van Maanen, Diagnostic assays applied in BVDV control in The Netherlands, 43-48, Copyright Elsevier 2005.